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Viral Haemorrhagic Septicaemia Virus

Search Sequence Reports Using BLAST


Location on first sequence (-I)
Location on second sequence (-J)
Expect: upper bound on the expected frequency of chance occurrence of a set of HSPs (-e)

Selectivity options

Wordsize (-W) (zero invokes default behavior)
Perform gapped alignment (-g)
X dropoff value for gapped alignment (in bits) (-X)
Cost to open a gap (-G)
Cost to extend a gap (-E)

Matrix (-M)

BLASTN Options

Penalty for a nucleotide mismatch (-q)
Reward for a nucleotide match (-r)
Query strands to search against database (-S) ? 1: top 2: bottom 3: both



Some explanations about the options

Expect: upper bound on the expected frequency of chance occurrence of a set of HSPs (-e)
The statistical significance threshold for reporting matches against database sequences; the default value is 10, such that 10 matches are expected to be found merely by chance, according to the stochastic model of Karlin and Altschul (1990). If the statistical significance ascribed to a match is greater than the EXPECT threshold, the match will not be reported. Lower EXPECT thresholds are more stringent, leading to fewer chance matches being reported. Fractional values are acceptable.

Selectivity options
X dropoff value for gapped alignment (in bits) (-X)
This is the value that control the path graph region explored by Blast during a gapped extension (Xg in the NAR paper) (default for blastp is 15).
Cost to open a gap (-G)
default is 5 for blastn, 10 for blastp
Cost to extend a gap (-E)
default is 2 for blastn, 1 for blastp
Limited values for gap existence and extension are supported for these three programs. Some supported and suggested values are:
Existence Extension
10 1
10 2
11 1
8 2
9 2
Sequence format
The sequence will be automatically converted in the format needed for the program
providing you enter a sequence either:
in plain (raw) sequence format or in one of the following known formats:
IG,GenBank,NBRF,EMBL,GCG,DNAStrider,Fitch,fasta,Phylip,PIR,MSF,ASN,PAUP,CLUSTALW
You may enter in the text area a database entry code, or an accession number, in this form:
database:entry_name
or:
database:accession.